Gills will play Colchester, Ipswich and Tottenham in the group stages of the Checkatrade Trophy.
The dates of Gillingham FC's EFL Trophy group stage fixtures have been confirmed.
Gills are in Group A of the southern section and have been drawn against Colchester, Ipswich and Spurs U21.
Two games will be played at MEMS Priestfield Stadium, with the other being contested at Portman Road against Ipswich Town.
Gillingham v Colchester United
Date: Tuesday 3 September (Priestfield)
Kick Off: 7.45pm
Ipswich Town v Gillingham
Date: Tuesday October 8 (Portman Road)
Kick-Off, 7.45pm
Gillingham v Tottenham U21
Date: Tuesday 12 November 2019 (Priestfield)
Kick Off: 7.45pm
Ticket prices for each fixture will be published in due course.